hidden
hidden

Umbilical Cord Blood Stem Cells Have Myogenic Potential

The replacement of the defective muscle through stem cells transplantation is a possible future treatment for patients suffering from Duchenne Muscular Dystrophy (DMD). The dystrophin gene, located at Xp21, codifies dystrophin, which is part of a protein complex responsible for the membrane stability of muscle cells. Its absence on muscle causes the severe Duchenne Muscular Dystrophy (DMD). A recent analysis showed the potential of CD34+ stem cells from umbilical cord blood to differentiate in muscle cells and express dystrophin. Protein expression was analyzed by Immunofluorescence, Western Blotting (WB) and Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR). CD34+ stem cells and myoblasts from a DMD affected patient started to fuse with muscle cells immediately after co-cultures establishment.

The replacement of the defective muscle through stem cells transplantation is a possible future treatment for patients suffering from Duchenne Muscular Dystrophy (DMD). The dystrophin gene, located at Xp21, codifies dystrophin, which is part of a protein complex responsible for the membrane stability of muscle cells. Its absence on muscle causes the severe Duchenne Muscular Dystrophy (DMD). A recent analysis showed the potential of CD34+ stem cells from umbilical cord blood to differentiate in muscle cells and express dystrophin. Protein expression was analyzed by Immunofluorescence, Western Blotting (WB) and Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR). CD34+ stem cells and myoblasts from a DMD affected patient started to fuse with muscle cells immediately after co-cultures establishment.

View Full Story